Author: Devon Chandler-Brown; Anna M. Bueno; Oguzhan Atay; David S. Tsao
Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Document date: 2020_4_10
ID: eui41zyg_26
Snippet: Primer and spike-in: Spike-in sequences were designed using the viral genomic region corresponding to the CDC designed N3 qPCR assay. Spike-in molecules have sequence identical to SARS-CoV-2 sequence (LC528232) including base positions 28216 to 29280 but lacking bases 28715-28718. Primers flanking the deletion were used for amplification. Sequencing was performed using a primer containing the forward amplification binding region......
Document: Primer and spike-in: Spike-in sequences were designed using the viral genomic region corresponding to the CDC designed N3 qPCR assay. Spike-in molecules have sequence identical to SARS-CoV-2 sequence (LC528232) including base positions 28216 to 29280 but lacking bases 28715-28718. Primers flanking the deletion were used for amplification. Sequencing was performed using a primer containing the forward amplification binding region.
Search related documents:
Co phrase search for related documents- base position and spike sequence: 1
- bind region and genomic region: 1
- bind region and SARS sequence: 1
- bind region and spike sequence: 1
- genomic region and qpcr assay: 1
- genomic region and SARS sequence: 1, 2, 3, 4
- qpcr assay and SARS sequence: 1, 2
- SARS sequence and spike molecule: 1
- SARS sequence and spike sequence: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76
- SARS sequence and spike spike sequence: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date