Author: Devon Chandler-Brown; Anna M. Bueno; Oguzhan Atay; David S. Tsao
Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Document date: 2020_4_10
ID: eui41zyg_26
Snippet: Primer and spike-in: Spike-in sequences were designed using the viral genomic region corresponding to the CDC designed N3 qPCR assay. Spike-in molecules have sequence identical to SARS-CoV-2 sequence (LC528232) including base positions 28216 to 29280 but lacking bases 28715-28718. Primers flanking the deletion were used for amplification. Sequencing was performed using a primer containing the forward amplification binding region......
Document: Primer and spike-in: Spike-in sequences were designed using the viral genomic region corresponding to the CDC designed N3 qPCR assay. Spike-in molecules have sequence identical to SARS-CoV-2 sequence (LC528232) including base positions 28216 to 29280 but lacking bases 28715-28718. Primers flanking the deletion were used for amplification. Sequencing was performed using a primer containing the forward amplification binding region.
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