Selected article for: "growth medium and post inoculation"
Author: Siying Ye; Chris Cowled; Cheng-Hon Yap; John Stambas
Title: Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1
  • Document date: 2018_5_17
    • ID: 6arj2i3m_28
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint with type IV human placenta collagen (0.05 mg/mL; Sigma) at 300,000 cells/cm 2 . Cells were then 398 cultured under liquid-covered conditions in a humidified incubator (5% CO 2 , 37°C) and growth 399 medium was changed every 48 hours. These culture conditions encourage ATII cells to grow and 400 differ.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint with type IV human placenta collagen (0.05 mg/mL; Sigma) at 300,000 cells/cm 2 . Cells were then 398 cultured under liquid-covered conditions in a humidified incubator (5% CO 2 , 37°C) and growth 399 medium was changed every 48 hours. These culture conditions encourage ATII cells to grow and 400 differentiate into alveolar epithelial cells. trypsin (Worthington) was included in media post-inoculation to assist replication. Virus titers were 420 determined using standard plaque assays using MDCK cells as previously described (56). 421 All rights reserved. No reuse allowed without permission.

    Search related documents:
    Co phrase search for related documents
    • ATII cell and post inoculation: 1
    • epithelial cell and MDCK cell: 1, 2, 3, 4, 5, 6
    • epithelial cell and plaque assay: 1, 2, 3, 4, 5, 6
    • epithelial cell and post inoculation: 1
    • epithelial cell and virus titer: 1, 2, 3
    • human placenta collagen and IV mg mL human placenta collagen: 1
    • human placenta collagen and mg mL human placenta collagen: 1
    • human placenta collagen and placenta collagen: 1
    • humidified incubator and plaque assay: 1
    • humidified incubator and virus titer: 1
    • IV mg mL human placenta collagen and mg mL human placenta collagen: 1
    • IV mg mL human placenta collagen and placenta collagen: 1