Author: Dene Littler; Benjamin Gully; Rhys N Colson; Jamie Rossjohn
Title: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9 Document date: 2020_3_30
ID: beoseizn_33
Snippet: Nsp9COV19 crystallized in 2.0-2.2M NH4SO4 and 0.1M Phosphate-citrate buffer pH 4.0. Crystals of the His-tag samples grew with rectangular morphology in space group P4122, however if the Histag was removed the crystals grew in space group P6122 form with hexagonal morphology. All diffraction data were collected at the Australian synchrotron's MX2 beamline at the Australian synchrotron ) (see Table 1 for details). Data were integrated in XDS (Kabsc.....
Document: Nsp9COV19 crystallized in 2.0-2.2M NH4SO4 and 0.1M Phosphate-citrate buffer pH 4.0. Crystals of the His-tag samples grew with rectangular morphology in space group P4122, however if the Histag was removed the crystals grew in space group P6122 form with hexagonal morphology. All diffraction data were collected at the Australian synchrotron's MX2 beamline at the Australian synchrotron ) (see Table 1 for details). Data were integrated in XDS (Kabsch, 2010) , processed with SCALA, phases were obtained through molecular replacement using PDB 1QZ8 (Egloff et al., 2004 ) as a search model. Subsequent rounds of manual building and refinement were performed in Coot and PHENIX (Liebschner et al., 2019) .
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