Selected article for: "affinity chromatography and molecular weight"
Author: Dene Littler; Benjamin Gully; Rhys N Colson; Jamie Rossjohn
Title: Crystal structure of the SARS-CoV-2 non-structural protein 9, Nsp9
  • Document date: 2020_3_30
    • ID: beoseizn_5
    Snippet: The Nsp9 protein from SARS-CoV-2 (Nsp9COV19) was cloned and recombinantly expressed in E. coli. The expression construct included an N-terminal Hexa-His tag attached via a rhinoviral 3Cprotease site. Following Ni-affinity chromatography Nsp9COV19 was further purified via size-exclusion chromatography to yield > 95% pure and homogeneous protein. Nsp9COV19 eluted from gel filtration columns with the apparent molecular weight of a dimer suggesting t.....
    Document: The Nsp9 protein from SARS-CoV-2 (Nsp9COV19) was cloned and recombinantly expressed in E. coli. The expression construct included an N-terminal Hexa-His tag attached via a rhinoviral 3Cprotease site. Following Ni-affinity chromatography Nsp9COV19 was further purified via size-exclusion chromatography to yield > 95% pure and homogeneous protein. Nsp9COV19 eluted from gel filtration columns with the apparent molecular weight of a dimer suggesting that, as with other Nsp9 proteins, Nsp9COV19 is an obligate homodimer. The Nterminal tag was removed prior to any biochemical experiments via overnight digestion with precision protease, as reported for Nsp9SARS (Sutton et al., 2004) .

    Search related documents:
    Co phrase search for related documents
    • expression construct and ni affinity: 1, 2, 3
    • expression construct and ni affinity chromatography: 1, 2
    • expression construct and size exclusion: 1, 2, 3, 4
    • expression construct and size exclusion chromatography: 1, 2, 3, 4
    • filtration column and gel filtration column: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
    • filtration column and molecular weight: 1, 2
    • filtration column and ni affinity: 1, 2
    • filtration column and size exclusion: 1, 2, 3, 4, 5, 6
    • filtration column and size exclusion chromatography: 1, 2, 3
    • filtration column and size exclusion chromatography purify: 1
    • gel filtration column and molecular weight: 1
    • gel filtration column and ni affinity: 1, 2
    • gel filtration column and size exclusion: 1, 2, 3, 4, 5
    • gel filtration column and size exclusion chromatography: 1, 2, 3
    • gel filtration column and size exclusion chromatography purify: 1
    • molecular weight and ni affinity: 1, 2, 3, 4, 5, 6, 7
    • molecular weight and ni affinity chromatography: 1, 2, 3, 4, 5, 6, 7
    • molecular weight and size exclusion: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • molecular weight and size exclusion chromatography: 1, 2, 3, 4, 5, 6, 7, 8