Selected article for: "PCR amplification and random PCR amplification"
Author: Palacios, Gustavo; Quan, Phenix-Lan; Jabado, Omar J.; Conlan, Sean; Hirschberg, David L.; Liu, Yang; Zhai, Junhui; Renwick, Neil; Hui, Jeffrey; Hegyi, Hedi; Grolla, Allen; Strong, James E.; Towner, Jonathan S.; Geisbert, Thomas W.; Jahrling, Peter B.; Büchen-Osmond, Cornelia; Ellerbrok, Heinz; Sanchez-Seco, Maria Paz; Lussier, Yves; Formenty, Pierre; Nichol, Stuart T.; Feldmann, Heinz; Briese, Thomas; Lipkin, W. Ian
Title: Panmicrobial Oligonucleotide Array for Diagnosis of Infectious Diseases
  • Cord-id: 0nl4cpn9
  • Document date: 2007_1_25
    • ID: 0nl4cpn9
    Snippet: To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, b
    Document: To facilitate rapid, unbiased, differential diagnosis of infectious diseases, we designed GreeneChipPm, a panmicrobial microarray comprising 29,455 sixty-mer oligonucleotide probes for vertebrate viruses, bacteria, fungi, and parasites. Methods for nucleic acid preparation, random primed PCR amplification, and labeling were optimized to allow the sensitivity required for application with nucleic acid extracted from clinical materials and cultured isolates. Analysis of nasopharyngeal aspirates, blood, urine, and tissue from persons with various infectious diseases confirmed the presence of viruses and bacteria identified by other methods, and implicated Plasmodium falciparum in an unexplained fatal case of hemorrhagic feverlike disease during the Marburg hemorrhagic fever outbreak in Angola in 2004–2005.

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