Author: Yuan, Yuan; Cao, Duanfang; Zhang, Yanfang; Ma, Jun; Qi, Jianxun; Wang, Qihui; Lu, Guangwen; Wu, Ying; Yan, Jinghua; Shi, Yi; Zhang, Xinzheng; Gao, George F.
Title: Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains Cord-id: 094lgjnn Document date: 2017_4_10
ID: 094lgjnn
Snippet: The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the except
Document: The envelope spike (S) proteins of MERS-CoV and SARS-CoV determine the virus host tropism and entry into host cells, and constitute a promising target for the development of prophylactics and therapeutics. Here, we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy. The overall structures resemble that from other coronaviruses including HKU1, MHV and NL63 reported recently, with the exception of the receptor binding domain (RBD). We captured two states of the RBD with receptor binding region either buried (lying state) or exposed (standing state), demonstrating an inherently flexible RBD readily recognized by the receptor. Further sequence conservation analysis of six human-infecting coronaviruses revealed that the fusion peptide, HR1 region and the central helix are potential targets for eliciting broadly neutralizing antibodies.
Search related documents:
Co phrase search for related documents- accurate rotational alignment and local resolution: 1
- acute sars cov respiratory syndrome coronavirus and local resolution: 1, 2, 3
- acute sars cov respiratory syndrome coronavirus and long helix: 1, 2, 3
- acute sars cov respiratory syndrome coronavirus and long trimer: 1
- acute sars cov respiratory syndrome coronavirus and low ph environment: 1, 2
- acute sars cov respiratory syndrome coronavirus and low resolution: 1, 2, 3, 4, 5
- local resolution and long trimer: 1
- local resolution and low resolution: 1, 2
- long central helix and low ph environment: 1, 2
- long central helix and low ph environment membrane fusion: 1
- long central helix and low ph environment membrane fusion activation: 1
- long helix and low ph environment: 1, 2
- long helix and low ph environment membrane fusion: 1
- long helix and low ph environment membrane fusion activation: 1
Co phrase search for related documents, hyperlinks ordered by date