Design of multi epitope-based peptide vaccine against E protein of human COVID-19: An immunoinformatics approach

Miyssa I. Abdelmageed; Abdelrahman H. Abdelmoneim; Mujahed I. Mustafa; Nafisa M. Elfadol; Naseem S. Murshed; Shaza W. Shantier; Abdelrafie M. Makhawi

The reference sequence of envelope protein was aligned with HCov-HKU1 reference protein using artemis comparison tool as illustrated in (Fig. 2 ). Figure 2 : Artemis analysis of envelope protein displaying 3 windows, the upper window represents HCov-HKU1 reference sequence and its genes are highlighted in blue starting from orflab gene and ending with N gene. The middle window describes the similarities and the difference between the two genomes. Red lines indicate match between genes from the two genomes blue lines indicates inversion which represents same sequences in the two genomes but they are organized in the opposite direction, and the lower windows represents COVID-19and its genes started from ARFLAB and ends with N genes.