Author: Piotrowski, Yvonne; Ponnusamy, Rajesh; Glaser, Stephanie; Daabach, Anniken; Moll, Ralf; Hilgenfeld, Rolf
Title: Production of Coronavirus Nonstructural Proteins in Soluble Form for Crystallization Cord-id: 0hfku8o4 Document date: 2007_11_28
ID: 0hfku8o4
Snippet: For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that ha
Document: For biophysical investigations on viral proteins, in particular for structure determination by X-ray crystallography, relatively large quantities of purified protein are necessary. However, expression of cDNAs coding for viral proteins in prokaryotic or eukaryotic systems is often not straightforward, and frequently the amount and/or the solubility of the protein obtained are not sufficient. Here, we describe a number of protocols for production of nonstructural proteins of coronaviruses that have proven to be efficient in increasing expression yields or solubilities.
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