Author: Asiedu Ebenezer
Title: Designing Effective small interfering RNA for Post-Transcriptional Silencing of Human GREM1: A Comprehensive Bioinformatics Approach Document date: 2020_1_24
ID: j99qg2a0_36
Snippet: Small interfering RNAs were designed to target human gremlin-1 using bioinformatics approach. Multiple computational algorithms were used to design potential siRNA candidates and the best candidates were selected based on empirical design rules and comprehensive target availability analysis. The selected candidates were then compared to experimentally . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyrigh.....
Document: Small interfering RNAs were designed to target human gremlin-1 using bioinformatics approach. Multiple computational algorithms were used to design potential siRNA candidates and the best candidates were selected based on empirical design rules and comprehensive target availability analysis. The selected candidates were then compared to experimentally . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.01.23.917559 doi: bioRxiv preprint functional siRNAs to suggest the efficacy of the designed siRNAs in this study. Out of 66 designed siRNA candidates, three were selected to be highly effective based on preferential sequence features for functional siRNAs, thermodynamics of siRNA duplexes and mRNA target availability. siRNA-6 (sense strand: 5'-CCAAGAAAUUCACUACCAU-3'), siRNA-7 (sense strand:
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