Cryo-EM structures reveal transcription initiation steps by yeast mitochondrial RNA polymerase

Brent De Wijngaert; Shemaila Sultana; Chhaya Dharia; Hans Vanbuel; Jiayu Shen; Daniel vasilchuk; Sergio E Martinez; Eaazhisai Kandiah; Smita S Patel; Kalyan Das

. CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint assembling y-mtIC; binding of UTPaS unwinds the downstream +3 base-pair and engages +3 template nucleotide base-paired with UTPaS to form a catalytic-competent quinary IC. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038620 doi: bioRxiv preprint Processing of y-mtIC micrographs and the roadmap for obtaining the final y-mtIC map was calculated at 3.7 Ã… resolution. The y-mtPIC particles grouped in the step 2 of 3D classification were processed to generate a density map at 3.5 Ã… resolution (not shown here) and map was confirmed to represent a state which is the same as the 3.1 Ã… y-mtPIC map. For y-mtPIC (C) and y-mtIC (D) structures, final density map from 3D refinement, FSC curve, angular distribution of the final set of particles, B-sharpened map color coded with estimated local resolution, and the B-factor puffy representation of the final model (blue