Author: Asif Shajahan; Nitin T. Supekar; Anne S. Gleinich; Parastoo Azadi
Title: Deducing the N- and O-glycosylation profile of the spike protein of novel coronavirus SARS-CoV-2 Document date: 2020_4_3
ID: f0xsisdg_6
Snippet: Here, we report the site-specific quantitative N-linked and O-linked glycan profiling on SARS-CoV-2 subunit S1 and S2 protein through glycoproteomics using high resolution LC-MS/MS. We used recombinant SARS-CoV-2 subunit S1 and S2 expressed in human cells, HEK 293, and observed partial N-glycan occupancy on 17 out of 22 N-glycosylation sites. We found that the remaining five N-glycosylation sites were unoccupied. Remarkably, we have unambiguously.....
Document: Here, we report the site-specific quantitative N-linked and O-linked glycan profiling on SARS-CoV-2 subunit S1 and S2 protein through glycoproteomics using high resolution LC-MS/MS. We used recombinant SARS-CoV-2 subunit S1 and S2 expressed in human cells, HEK 293, and observed partial N-glycan occupancy on 17 out of 22 N-glycosylation sites. We found that the remaining five N-glycosylation sites were unoccupied. Remarkably, we have unambiguously identified 2 unexpected O-glycosylation sites at the receptor binding domain (RBD) of subunit S1.
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