Author: Siying Ye; Chris Cowled; Cheng-Hon Yap; John Stambas
Title: Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1 Document date: 2018_5_17
ID: 6arj2i3m_17
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint MOIs of 0.5 and 1 and were higher at 48 hpi when compared to 24 hpi (Fig. 3F ). HPAI H5N1 virus 230 infection at MOIs of 0.5, 1 and 2 stimulated higher endogenous levels of CEACAM1 protein 231 expression when compared to low pathogenic PR8 H1N1 virus infection at a MOI of 2 (a maximum 232 23-fold increa.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint MOIs of 0.5 and 1 and were higher at 48 hpi when compared to 24 hpi (Fig. 3F ). HPAI H5N1 virus 230 infection at MOIs of 0.5, 1 and 2 stimulated higher endogenous levels of CEACAM1 protein 231 expression when compared to low pathogenic PR8 H1N1 virus infection at a MOI of 2 (a maximum 232 23-fold increase induced by H5N1 at MOIs of 0.5 and 1, 48 hpi when compared to PR8 at MOI of 2), 233 suggesting a possible role for CEACAM1 in influenza virus pathogenicity (Fig. 3F) . (data not shown). CEACAM1 protein expression was reduced by approximately 50% in both ATII and 250 A549 cells following siCEACAM1 transfection when compared with siNeg-transfected cells (Fig. 4C) . 251
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