Author: Siying Ye; Chris Cowled; Cheng-Hon Yap; John Stambas
Title: Deep sequencing of primary human lung epithelial cells challenged with H5N1 influenza virus reveals a proviral role for CEACAM1 Document date: 2018_5_17
ID: 6arj2i3m_29
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint RNA extraction, quality control (QC) and HiSeq analysis. ATII cells from three individual 422 donors were used for HiSeq analysis. Total RNA was extracted from cells using a RNeasy Mini kit 423 (Qiagen). Influenza-infected cells were washed with PBS three times and cells lysed with RLT buffer 424 supple.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/324723 doi: bioRxiv preprint RNA extraction, quality control (QC) and HiSeq analysis. ATII cells from three individual 422 donors were used for HiSeq analysis. Total RNA was extracted from cells using a RNeasy Mini kit 423 (Qiagen). Influenza-infected cells were washed with PBS three times and cells lysed with RLT buffer 424 supplemented with β-mercaptoethanol (10 μL/mL; Gibco). Cell lysates were homogenized with 425
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