Selected article for: "assay validation and qrt PCR assay"
Author: Minghua Jiang; Wenjie Fang; Amir Aratehfar; Xiaojing Li; Liyan ling; Hua Fang; Farnaz Farnaz Daneshnia; Jian Yu; Wanqing Liao; Hao Pei; Weihua Pan; Cornelia Lass-Florl
Title: Development and validation of a rapid single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable and high-throughput screening of COVID-19
  • Document date: 2020_3_20
    • ID: eo2pcgix_22
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 time to positivity by our RT-LAMP assay and the Ct values reported by qRT-PCR when using 168 clinical samples. Therefore, we considered our assay a qualitative one. This fact will show that the 169 analytical valuation should be always accompanied by clinical validation to observe the real 170 capabilities of a given assay an.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https: //doi.org/10.1101 //doi.org/10. /2020 time to positivity by our RT-LAMP assay and the Ct values reported by qRT-PCR when using 168 clinical samples. Therefore, we considered our assay a qualitative one. This fact will show that the 169 analytical valuation should be always accompanied by clinical validation to observe the real 170 capabilities of a given assay and that the results obtained in analytical evaluation step are not always 171 reflected in real-life. 172

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