Author: Minghua Jiang; Wenjie Fang; Amir Aratehfar; Xiaojing Li; Liyan ling; Hua Fang; Farnaz Farnaz Daneshnia; Jian Yu; Wanqing Liao; Hao Pei; Weihua Pan; Cornelia Lass-Florl
Title: Development and validation of a rapid single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable and high-throughput screening of COVID-19 Document date: 2020_3_20
ID: eo2pcgix_4
Snippet: Most recently a newer generation of single step RT-LAMP were developed to detect SARS COV-2, 65 but these assays were not challenged with real clinical samples obtained from COVID-19 positive 66 patients (5, 6). Therefore, we developed a sensitive, specific, and rapid RT-LAMP assay and its 67 performance was challenged by an extensive number of confirmed COVID-19 (n=47) and negative 68 patients (n=213) relative to qRT-PCR assays approved by two C.....
Document: Most recently a newer generation of single step RT-LAMP were developed to detect SARS COV-2, 65 but these assays were not challenged with real clinical samples obtained from COVID-19 positive 66 patients (5, 6). Therefore, we developed a sensitive, specific, and rapid RT-LAMP assay and its 67 performance was challenged by an extensive number of confirmed COVID-19 (n=47) and negative 68 patients (n=213) relative to qRT-PCR assays approved by two Chinese Food and Drug 69 Administration (qRT-PCR NMPA). Altogether, we present a rapid and reliable diagnostic tool that 70 potentially could be deployed for high-throughput screening applications in referral and local 71 laboratories. 72
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