Selected article for: "detection limit and RT LAMP detection limit"
Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
  • Document date: 2020_3_12
    • ID: 1qn5y4gc_33
    Snippet: The limit of detection of RT-LAMP assays suggested in this study, 100 copies per reaction, may not enough for sensitive screening of suspicious patients. This relatively high limit of detection would be from the target sequences used for primer design that are selected by specificity versus SARS-CoV. Indeed, target GC percentage and Tm of primers had to be adjusted to get enough number of starting sets for primer screening during LAMP primer desi.....
    Document: The limit of detection of RT-LAMP assays suggested in this study, 100 copies per reaction, may not enough for sensitive screening of suspicious patients. This relatively high limit of detection would be from the target sequences used for primer design that are selected by specificity versus SARS-CoV. Indeed, target GC percentage and Tm of primers had to be adjusted to get enough number of starting sets for primer screening during LAMP primer design to give less-optimal primer sets.

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