A score combining early detection of cytokines accurately predicts COVID-19 severity and intensive care unit transfer

Nagant, Carole; Ponthieux, Fanny; Smet, Julie; Dauby, Nicolas; Doyen, Virginie; Besse-Hammer, Tatiana; De Bels, David; Maillart, Evelyne; Corazza, Francis

Objectives We aimed to explore cytokine profile in patients related to Coronavirus Disease 2019 (COVID-19) severity and to establish a predictive cytokine score to discriminate severe from non-severe cases and provide a prognosis parameter for patients that will require intensive care unit (ICU) transfer. Methods Serum samples of 63 patients diagnosed with SARS-CoV-2 infection were collected early after hospital admission (day 0-3). Patients were categorized in five groups based on the clinical presentation, the PaO2/FiO2 ratio and the requirement of mechanical ventilation. Results Three cytokines, IL-6, IL-8 and IL-10 were markedly higher in severe forms (n = 44) than in non-severe forms (n = 19) (p < 0.005). A score combining levels of these three cytokines (IL-6*IL-8*IL-10) had the highest performance to predict severity: sensitivity of 86.4% (95% CI, 72.4-94.8) and specificity of 94.7% (95% CI, 74.0-99.9) for a cutoff value of 2068 pg/mL. Elevated levels of IL-6, IL-8 and IL-10 were also found in critically ill patients. The combination of IL-6*IL-10 serum levels allowed the highest predictability for ICU transfer: AUC of 0.898 (p < 0.0001). Conclusion The combinatorial IL-6*IL-8*IL-10 score at presentation was highly predictive of the progression to a severe form of the disease and could contribute to improve patients triage and to adapt therapeutic strategy within clinical trials more accurately and efficiently.