Author: Poon, Leo L.M; Wong, Bonnie W.Y; Chan, Kwok Hung; Leung, Cynthia S.W; Yuen, Kwok Yung; Guan, Yi; Peiris, Joseph S.M
Title: A one step quantitative RT-PCR for detection of SARS coronavirus with an internal control for PCR inhibitors Cord-id: 11fdzjpv Document date: 2004_2_3
ID: 11fdzjpv
Snippet: In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal
Document: In this study, we report a one step quantitative RT-PCR assay for severe acute respiratory syndrome (SARS) diagnosis. The overall detection rate for clinical samples collected from Days 1 to 9 of disease onset is 86.2% and the specificity of the assay is 100%. To detect false negative results due to PCR inhibitors or faulty RNA extraction, we have further modified this one step RT-PCR assay for simultaneous detection of severe acute respiratory syndrome (SARS) coronavirus (CoV) and 18S ribosomal RNA (rRNA) as an internal positive control.
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