Designing Effective small interfering RNA for Post-Transcriptional Silencing of Human GREM1: A Comprehensive Bioinformatics Approach

Asiedu Ebenezer

Gremlin-1 primary transcript containing 14575 nucleotides was analyzed with the ExPASy translation tool. The open reading frame (ORF) that codes for the human gremlin-1 was predicted to be in 5' 3' Frame 1 (Supplementary Fig. 1 ). The ORF was observed to be continuous stretch of nucleotides starting from position 160 to 757 of the primary transcript ( Supplementary Fig. 2) . Several in-silico tools were used to design siRNA candidates that target gremlin-1 mRNA, precisely the ORF sequence. Sixty-six potential siRNAs were designed to silence gremlin-1 (Supplementary Table 1 Number of common base pairs between MFE and Ensemble centroid (base pair in green).