Selected article for: "differential expression analysis and mrna seq"
Author: Yuan, Shuai; Peng, Lei; Park, Jonathan J.; Hu, Yingxia; Devarkar, Swapnil C.; Dong, Matthew B.; Shen, Qi; Wu, Shenping; Chen, Sidi; Lomakin, Ivan B.; Xiong, Yong
Title: Nonstructural Protein 1 of SARS-CoV-2 Is a Potent Pathogenicity Factor Redirecting Host Protein Synthesis Machinery toward Viral RNA
  • Cord-id: 0fqx6ee9
  • Document date: 2020_12_17
    • ID: 0fqx6ee9
    Snippet: The causative virus of the COVID-19 pandemic, SARS-CoV-2, uses its nonstructural protein 1 (Nsp1) to suppress cellular, but not viral, protein synthesis through yet unknown mechanisms. We show here that among all viral proteins, Nsp1 has the largest impact on host viability in the cells of human lung origin. Differential expression analysis of mRNA-seq data revealed that Nsp1 broadly alters the cellular transcriptome. Our cryo-EM structure of the Nsp1-40S ribosome complex shows that Nsp1 inhibit
    Document: The causative virus of the COVID-19 pandemic, SARS-CoV-2, uses its nonstructural protein 1 (Nsp1) to suppress cellular, but not viral, protein synthesis through yet unknown mechanisms. We show here that among all viral proteins, Nsp1 has the largest impact on host viability in the cells of human lung origin. Differential expression analysis of mRNA-seq data revealed that Nsp1 broadly alters the cellular transcriptome. Our cryo-EM structure of the Nsp1-40S ribosome complex shows that Nsp1 inhibits translation by plugging the mRNA entry channel of the 40S. We also determined the structure of the 48S preinitiation complex formed by Nsp1, 40S, and the cricket paralysis virus internal ribosome entry site (IRES) RNA, which shows that it is nonfunctional because of the incorrect position of the mRNA 3′ region. Our results elucidate the mechanism of host translation inhibition by SARS-CoV-2 and advance understanding of the impacts from a major pathogenicity factor of SARS-CoV-2.

    Search related documents:
    Co phrase search for related documents
    • accessory structural and local environment: 1
    • accessory structural and luciferase reporter: 1
    • acute sars cov respiratory syndrome coronavirus and local environment: 1, 2, 3, 4
    • acute sars cov respiratory syndrome coronavirus and local resolution: 1, 2, 3
    • acute sars cov respiratory syndrome coronavirus and long term illness: 1, 2, 3, 4, 5
    • acute sars cov respiratory syndrome coronavirus and luciferase reporter: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18
    • acute sars cov respiratory syndrome coronavirus and luciferase signal: 1