Author: Valeria Lulla; Andrew E. Firth
Title: A hidden gene in astroviruses encodes a cell-permeabilizing protein involved in virus release Document date: 2019_6_6
ID: avq3zwmc_47
Snippet: Ribo-Seq analysis was performed as described previously 14 . Adaptor sequences were trimmed using the FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit) and trimmed reads shorter than 25 nt were discarded. Reads were mapped to host (Homo sapiens) and virus RNA using bowtie version 1 39 , with parameters -v 2 --best (i.e. maximum 2 mismatches, report best match)......
Document: Ribo-Seq analysis was performed as described previously 14 . Adaptor sequences were trimmed using the FASTX-Toolkit (http://hannonlab.cshl.edu/fastx_toolkit) and trimmed reads shorter than 25 nt were discarded. Reads were mapped to host (Homo sapiens) and virus RNA using bowtie version 1 39 , with parameters -v 2 --best (i.e. maximum 2 mismatches, report best match).
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