Author: Minghua Jiang; Wenjie Fang; Amir Aratehfar; Xiaojing Li; Liyan ling; Hua Fang; Farnaz Farnaz Daneshnia; Jian Yu; Wanqing Liao; Hao Pei; Weihua Pan; Cornelia Lass-Florl
Title: Development and validation of a rapid single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable and high-throughput screening of COVID-19 Document date: 2020_3_20
ID: eo2pcgix_10
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10. 1101 Signal intensity and the time to obtain decent amplification curves were recorded and Institute of Medical Mycology, Shanghai Changzheng hospital). LAMP incubation time was set to 60 109 minutes to observe both limit of detection and cross-reactivity (LAMP conditions are mentioned in 110 clinical evaluation section). The reaction endpoint time w.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10. 1101 Signal intensity and the time to obtain decent amplification curves were recorded and Institute of Medical Mycology, Shanghai Changzheng hospital). LAMP incubation time was set to 60 109 minutes to observe both limit of detection and cross-reactivity (LAMP conditions are mentioned in 110 clinical evaluation section). The reaction endpoint time was set in a way to detect the lowest possible 111 copy number of virus without any cross-reaction. 112
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