Author: Devon Chandler-Brown; Anna M. Bueno; Oguzhan Atay; David S. Tsao
Title: A Highly Scalable and Rapidly Deployable RNA Extraction-Free COVID-19 Assay by Quantitative Sanger Sequencing Document date: 2020_4_10
ID: eui41zyg_38
Snippet: For concordance calls, Sanger sequencing was analyzed using the following procedure. The primary base sequence based on automatic calling were aligned to the viral genome. If the aligned sequence matched the viral genomic sequence without any deletion, then the sample is called positive for viral RNA. If the sequence does not match the reference, then the signal to noise ratio is checked with any less than 500 indicating insufficient signal which.....
Document: For concordance calls, Sanger sequencing was analyzed using the following procedure. The primary base sequence based on automatic calling were aligned to the viral genome. If the aligned sequence matched the viral genomic sequence without any deletion, then the sample is called positive for viral RNA. If the sequence does not match the reference, then the signal to noise ratio is checked with any less than 500 indicating insufficient signal which returns an indeterminate result. If signal to noise is greater than 500, then the ratio of genomic sequence to spike-in sequence is quantified by performing robust linear regression of genomic peak heights to spike-in peak heights.
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