Selected article for: "assay detect and predictive value"
Author: Minghua Jiang; Wenjie Fang; Amir Aratehfar; Xiaojing Li; Liyan ling; Hua Fang; Farnaz Farnaz Daneshnia; Jian Yu; Wanqing Liao; Hao Pei; Weihua Pan; Cornelia Lass-Florl
Title: Development and validation of a rapid single-step reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) system potentially to be used for reliable and high-throughput screening of COVID-19
  • Document date: 2020_3_20
    • ID: eo2pcgix_21
    Snippet: Subsequently, our RT-LAMP assay showed the sensitivity, specificity, negative predictive value, and 162 positive predictive value of 91.4%, 99.5%, 98.1%, and 97.7%, respectively (Supplementary Table 2 163 and 3). The fact that our assay could not detect four positive patients was owing to using 2.5 less 164 RNA input (2 µl) relative to qRT-PCR (5 µl). In the future, we will try to use various RNA input 165 volume (5, 8, and 10 µl) to observe .....
    Document: Subsequently, our RT-LAMP assay showed the sensitivity, specificity, negative predictive value, and 162 positive predictive value of 91.4%, 99.5%, 98.1%, and 97.7%, respectively (Supplementary Table 2 163 and 3). The fact that our assay could not detect four positive patients was owing to using 2.5 less 164 RNA input (2 µl) relative to qRT-PCR (5 µl). In the future, we will try to use various RNA input 165 volume (5, 8, and 10 µl) to observe if we could obtain a higher sensitivity. Although our RT-LAMP 166 assay was developed to be quantitative, we could not find any pattern and association between the 167 All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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