Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone

Sanchita Bhadra; Timothy E. Riedel; Miguel A. Saldaña; Shivanand Hegde; Nicole Pederson; Grant L. Hughes; Andrew D. Ellington

The paired results of morphological identification and LAMP-OSD analysis were compared using 241 2x2 contingency tables. Sensitivity or true positive rate was calculated by using the formula 242 The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. With a single endpoint visual 'yes/no' readout of OSD fluorescence (either directly observed or 277 imaged using cellphone camera), the Ae. aegypti coi LAMP-OSD assay could reliably identify 278 the presence of as few as 4 copies of synthetic target DNA (Fig 1) . Similarly, the cellphone-279 imaged wsp LAMP-OSD assay produced bright visible fluorescence when presented with only 280 All rights reserved. No reuse allowed without permission.