Author: Armin Ensser; Klaus Ueberla
Title: Determination of daily reproduction numbers of SARS-CoV2 based on death cases suggests more rapid initial spread in Italy and the United States Document date: 2020_3_31
ID: 4izymiy4_18
Snippet: In many cases, it is revealing to express integration rates as a ratio of the mean number of proviruses per target cell. To determine this ratio, the assay can be expanded to directly detect cell numbers. Kinetic PCR is performed for â¤-globin DNA. The standard(s) used for this assay must match the unknowns in terms of ploidy. For example, if (euploid) primary T cells are being studied, an appropriate standard would be graded doses of genomic DN.....
Document: In many cases, it is revealing to express integration rates as a ratio of the mean number of proviruses per target cell. To determine this ratio, the assay can be expanded to directly detect cell numbers. Kinetic PCR is performed for â¤-globin DNA. The standard(s) used for this assay must match the unknowns in terms of ploidy. For example, if (euploid) primary T cells are being studied, an appropriate standard would be graded doses of genomic DNA from (euploid) peripheral blood mononuclear cells, while an infected aneuploid tumor cell line should be compared to a standard prepared from matched uninfected cells.
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