Selected article for: "analytical sensitivity and serial dilution"
Author: Tieying Hou; Weiqi Zeng; Minling Yang; Wenjing Chen; Lili Ren; Jingwen Ai; Ji Wu; Yalong Liao; Xuejing Gou; Yongjun Li; Xiaorui Wang; Hang Su; Jianwei Wang; Bing Gu; Teng Xu
Title: Development and Evaluation of A CRISPR-based Diagnostic For 2019-novel Coronavirus
  • Document date: 2020_2_23
    • ID: hth8f5sn_15
    Snippet: Based on the three potential target sequences we identified, multiple sets of RPA primers 197 and CRISPR gRNAs were designed and screened. Among these, the set that targeted Orf1ab 198 showed the best overall performance of sensitivity and specificity, and therefore, was used to 199 develop CRISPR-nCoV in this study for further evaluation (data not shown). 200 We then sought to determine its analytical sensitivity by serial dilution at various 20.....
    Document: Based on the three potential target sequences we identified, multiple sets of RPA primers 197 and CRISPR gRNAs were designed and screened. Among these, the set that targeted Orf1ab 198 showed the best overall performance of sensitivity and specificity, and therefore, was used to 199 develop CRISPR-nCoV in this study for further evaluation (data not shown). 200 We then sought to determine its analytical sensitivity by serial dilution at various 201 concentrations. As shown in Figure 2B , CRISPR-nCoV consistently detected 7.5 copies of 202 2019-nCoV in all 10 replicates, 2.5 copies in 6 out of 10 , and 1.25 copies in 2 out of 10 runs.

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