Selected article for: "copy number and data point"
Author: Tieying Hou; Weiqi Zeng; Minling Yang; Wenjing Chen; Lili Ren; Jingwen Ai; Ji Wu; Yalong Liao; Xuejing Gou; Yongjun Li; Xiaorui Wang; Hang Su; Jianwei Wang; Bing Gu; Teng Xu
Title: Development and Evaluation of A CRISPR-based Diagnostic For 2019-novel Coronavirus
  • Document date: 2020_2_23
    • ID: hth8f5sn_10
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is For the evaluation of LoD by the number of DNA copies, DNA of the 2019-nCoV plasmid 155 was purified and the concentration was determined by a Qubit (Thermo Fisher, 156 Massachusetts). The copy number concentration was then calculated based the weight and the 157 length of fragment. Serial dilution with nuclease-free water was done to achieve desired 158 concentrations. 2.5μ.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is For the evaluation of LoD by the number of DNA copies, DNA of the 2019-nCoV plasmid 155 was purified and the concentration was determined by a Qubit (Thermo Fisher, 156 Massachusetts). The copy number concentration was then calculated based the weight and the 157 length of fragment. Serial dilution with nuclease-free water was done to achieve desired 158 concentrations. 2.5μl of extracted DNA at each titer was used as templates. Ten replicates 159 were performed at each data point near detection limit. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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