Author: Tieying Hou; Weiqi Zeng; Minling Yang; Wenjing Chen; Lili Ren; Jingwen Ai; Ji Wu; Yalong Liao; Xuejing Gou; Yongjun Li; Xiaorui Wang; Hang Su; Jianwei Wang; Bing Gu; Teng Xu
Title: Development and Evaluation of A CRISPR-based Diagnostic For 2019-novel Coronavirus Document date: 2020_2_23
ID: hth8f5sn_10
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is For the evaluation of LoD by the number of DNA copies, DNA of the 2019-nCoV plasmid 155 was purified and the concentration was determined by a Qubit (Thermo Fisher, 156 Massachusetts). The copy number concentration was then calculated based the weight and the 157 length of fragment. Serial dilution with nuclease-free water was done to achieve desired 158 concentrations. 2.5μ.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is For the evaluation of LoD by the number of DNA copies, DNA of the 2019-nCoV plasmid 155 was purified and the concentration was determined by a Qubit (Thermo Fisher, 156 Massachusetts). The copy number concentration was then calculated based the weight and the 157 length of fragment. Serial dilution with nuclease-free water was done to achieve desired 158 concentrations. 2.5μl of extracted DNA at each titer was used as templates. Ten replicates 159 were performed at each data point near detection limit. the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
Search related documents:
Co phrase search for related documents- copy number and detection limit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20
- copy number and dna copy: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45
- copy number and dna copy number: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41
- copy number and extract dna: 1
- copy number and serial dilution: 1, 2
- copy number concentration and detection limit: 1, 2
- data point and detection limit: 1, 2
- detection limit and dna copy: 1, 2, 3, 4
- detection limit and dna copy number: 1
- detection limit and extract dna: 1, 2
- detection limit and lod evaluation: 1, 2, 3, 4, 5
- detection limit and nuclease free water: 1, 2, 3
- detection limit and serial dilution: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17
- dna copy and serial dilution: 1, 2
- dna copy number and serial dilution: 1
- nuclease free water and serial dilution: 1
- nuclease free water serial dilution and serial dilution: 1
Co phrase search for related documents, hyperlinks ordered by date