Selected article for: "high throughput and sample type"

Author: Finoketti, Fernando; dos Santos, Raíssa Nunes; Campos, Aline Alves Scarpellini; Zani, André Luís da Silva; Barboza, Camila Mosca; Fernandes, Marcélia Emanuele Sad; de Souza, Tatiane de Cassia Pardo; dos Santos, Driele Delanira; Bortolanza, Giovana Werneck; Filho, Henrique Ortêncio; Roehe, Paulo Michel; Franco, Ana Cláudia; de Carvalho Ruthner Batista, Helena Beatriz
Title: Detection of adenovirus, papillomavirus and parvovirus in Brazilian bats of the species Artibeus lituratus and Sturnira lilium
  • Cord-id: 1uijeo9o
  • Document date: 2019_2_10
  • ID: 1uijeo9o
    Snippet: Bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. The diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. Therefore, the aim of this study was to detect fragments of viral genome
    Document: Bats play a significant role in maintaining their ecosystems through pollination, dispersal of seeds, and control of insect populations, but they are also known to host many microorganisms and have been described as natural reservoirs for viruses with zoonotic potential. The diversity of viruses in these animals remains largely unknown, however, because studies are limited by species, location, virus target, or sample type. Therefore, the aim of this study was to detect fragments of viral genomes in bat samples. We performed high-throughput sequencing analysis and specific PCR and RT-PCR on pools of anal and oropharyngeal swabs from Artibeus lituratus and Sturnira lilium collected in southern Brazil. As a result, a member of the family Adenoviridae related to human adenovirus C was detected in anal swabs from S. lilium. In addition, we detected a papillomavirus in an anal swab from A. lituratus. Our analyses also allowed the detection of adenoviruses and parvoviruses in oropharyngeal swabs collected from A. lituratus. These results increase our knowledge about viral diversity and illustrate the importance of conducting virus surveillance in bats.

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