Author: Christine Dahlke; Jasmin Heidepriem; Robin Kobbe; Rene Santer; Till Koch; Anahita Fathi; My L. Ly; Stefan Schmiedel; Peter H. Seeberger; Marylyn M. Addo; Felix F. Loeffler
Title: Distinct early IgA profile may determine severity of COVID-19 symptoms: an immunological case series Document date: 2020_4_17
ID: icl1t9d6_14
Snippet: The whole proteome of SARS-CoV-2 (GenBank ID: MN908947.3) was mapped as 4 883 spots of overlapping 15-mer peptides with a lateral shift of two AA on peptide microarrays, obtained from PEPperPRINT GmbH (Heidelberg, Germany). Patient sera were diluted 1:200 and incubated on the arrays overnight. Afterwards, IgG, IgM, and IgA serum antibody interactions were differentially detected with fluorescently labeled secondary antibodies. For details, see Su.....
Document: The whole proteome of SARS-CoV-2 (GenBank ID: MN908947.3) was mapped as 4 883 spots of overlapping 15-mer peptides with a lateral shift of two AA on peptide microarrays, obtained from PEPperPRINT GmbH (Heidelberg, Germany). Patient sera were diluted 1:200 and incubated on the arrays overnight. Afterwards, IgG, IgM, and IgA serum antibody interactions were differentially detected with fluorescently labeled secondary antibodies. For details, see Supporting Information. All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
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