Selected article for: "coli expression and expression vector"

Author: Tieying Hou; Weiqi Zeng; Minling Yang; Wenjing Chen; Lili Ren; Jingwen Ai; Ji Wu; Yalong Liao; Xuejing Gou; Yongjun Li; Xiaorui Wang; Hang Su; Jianwei Wang; Bing Gu; Teng Xu
Title: Development and Evaluation of A CRISPR-based Diagnostic For 2019-novel Coronavirus
  • Document date: 2020_2_23
  • ID: hth8f5sn_6
    Snippet: Cas13a ORF was then cloned into expression vector Pc013 and transfected into E. 116 coli BL21, which were first grown at 37°C and incubated with IPTG at 16°C. Proteins were the author/funder, who has granted medRxiv a license to display the preprint in perpetuity......
    Document: Cas13a ORF was then cloned into expression vector Pc013 and transfected into E. 116 coli BL21, which were first grown at 37°C and incubated with IPTG at 16°C. Proteins were the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.

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