Selected article for: "primer set and specific primer set"
Author: Gun-Soo Park; Keunbon Ku; Seung-Hwa Beak; Seong Jun Kim; Seung Il Kim; Bum-Tae Kim; Jin-Soo Maeng
Title: Development of Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) Assays Targeting SARS-CoV-2
  • Document date: 2020_3_12
    • ID: 1qn5y4gc_30
    Snippet: Our RT-LAMP assays showed specificity to SARS-CoV-2 versus alphacoronavirus (hCoV-229E), betacoronavirus (hCoV-OC43) and MERS-CoV. Although we could not test specificity to SARS-CoV because proper sample was not in our hand, specificity of the RT-LAMP assays are easily expectable from the mismatching bases in primer binding sites (Figure 1) . Especially, both of F1/B1 sites of "Nsp3_1-61" primer set is in SARS-CoV-2 specific region of which align.....
    Document: Our RT-LAMP assays showed specificity to SARS-CoV-2 versus alphacoronavirus (hCoV-229E), betacoronavirus (hCoV-OC43) and MERS-CoV. Although we could not test specificity to SARS-CoV because proper sample was not in our hand, specificity of the RT-LAMP assays are easily expectable from the mismatching bases in primer binding sites (Figure 1) . Especially, both of F1/B1 sites of "Nsp3_1-61" primer set is in SARS-CoV-2 specific region of which aligning SARS-CoV sequence is not exist.

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