Author: Roberto Balbontín; Nelson Frazão; Isabel Gordo
Title: DNA breaks-mediated cost reveals RNase HI as a new target for selectively eliminating antibiotic resistance Document date: 2019_9_5
ID: 5hfenevk_4
Snippet: RpoC Q1126K (40) . As hypothesized, both cost and SOS induction are greatly reduced in the compensated strain (Figure 2A ), confirming that DNA breaks are targeted by compensatory evolution. In order to test if this is general, we analyzed nine compensated clones from three independent populations of the RpsL K43T RpoB H526Y double mutant propagated for fifteen days in the absence of antibiotics. As expected, the costs are smaller in the evolved .....
Document: RpoC Q1126K (40) . As hypothesized, both cost and SOS induction are greatly reduced in the compensated strain (Figure 2A ), confirming that DNA breaks are targeted by compensatory evolution. In order to test if this is general, we analyzed nine compensated clones from three independent populations of the RpsL K43T RpoB H526Y double mutant propagated for fifteen days in the absence of antibiotics. As expected, the costs are smaller in the evolved strains and, as hypothesized, all the nine compensated clones show decreased SOS induction compared to their resistant ancestor ( Figure 2B ). This demonstrates that compensatory evolution widely targets mechanisms that reduce DNA breaks and further reinforces the notion that DNA breaks are major contributors to the cost of antibiotic resistance.
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