Selected article for: "background signal reduce and CDC assay"
Author: Yosuke Hirotsu; Hitoshi Mochizuki; Masao Omata
Title: Double-Quencher Probes Improved the Detection Sensitivity of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) by One-Step RT-PCR
  • Document date: 2020_3_20
    • ID: 2gokv7id_15
    Snippet: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037903 doi: medRxiv preprint assays target two sites (N1 and N2) (Figure 1 ). Previous study showed the mismatch of reverse primer in the N1 of the NIID assay with the sequence in the current database because it was constructed based on another reported sequence (GenBank: MN908947.1) [14] . In addition, this mismatch in the reverse primers had no influence on the detect.....
    Document: The copyright holder for this preprint . https://doi.org/10.1101/2020.03.17.20037903 doi: medRxiv preprint assays target two sites (N1 and N2) (Figure 1 ). Previous study showed the mismatch of reverse primer in the N1 of the NIID assay with the sequence in the current database because it was constructed based on another reported sequence (GenBank: MN908947.1) [14] . In addition, this mismatch in the reverse primers had no influence on the detection sensitivity compared to the perfectmatched reverse primer stated in the previous report [14] . CDC and NIID assays contain single-quencher probes in each assay; however, we originally generated the double-quencher technology in YCH assays to reduce background signal.

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