Author: Jonathon A. Siva-Jothy; Pedro F. Vale
Title: Dissecting genetic and sex-specific host heterogeneity in pathogen transmission potential Document date: 2019_8_14
ID: 0v5q4kp9_3
Snippet: Following phase separation, the upper aqueous layer was removed from each 178 sample and added to 25μl of isopropanol, tubes were then inverted twice, before 179 being centrifuged at 12,000×g for 10 minutes at 4°C. Precipitated RNA was then 180 washed by removing the supernatant, and re-dissolving the RNA pellet in 50μl of 75% 181 ethanol before being centrifuged at 7,500×g for 5 minutes at 4°C. RNA suspension 182 was achieved by removing 4.....
Document: Following phase separation, the upper aqueous layer was removed from each 178 sample and added to 25μl of isopropanol, tubes were then inverted twice, before 179 being centrifuged at 12,000×g for 10 minutes at 4°C. Precipitated RNA was then 180 washed by removing the supernatant, and re-dissolving the RNA pellet in 50μl of 75% 181 ethanol before being centrifuged at 7,500×g for 5 minutes at 4°C. RNA suspension 182 was achieved by removing 40μl of the ethanol supernatant, allowing the rest to dry 183 by evaporation and dissolving the remaining RNA pellet in 20μl of RNase-free water. 184
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