Author: Prischich, Davia; del Dedo, Javier Encinar; Cambra, Maria; Prat, Judit; Camarero, Nuria; Nevola, Laura; MartÃn-Quirós, Andrés; Rebollo, Elena; Giralt, Ernest; Isabel Geli, MarÃa; Gorostiza, Pau
Title: Light-dependent inhibition of clathrin-mediated endocytosis in yeast Cord-id: 1rdnxnuc Document date: 2021_4_1
ID: 1rdnxnuc
Snippet: Clathrin-mediated endocytosis (CME) is an essential cellular process, which is evolutionarily conserved among eukaryotes. Yeast constitutes a powerful genetic model to dissect the complex endocytic machinery, yet there is a lack of pharmacological agents that could complement genetics in selectively and reversibly interfere with CME in these organisms. TL2 is a light-regulated peptide inhibitor that targets the AP2/β-arrestin interaction and that can photocontrol CME with high spatiotemporal pr
Document: Clathrin-mediated endocytosis (CME) is an essential cellular process, which is evolutionarily conserved among eukaryotes. Yeast constitutes a powerful genetic model to dissect the complex endocytic machinery, yet there is a lack of pharmacological agents that could complement genetics in selectively and reversibly interfere with CME in these organisms. TL2 is a light-regulated peptide inhibitor that targets the AP2/β-arrestin interaction and that can photocontrol CME with high spatiotemporal precision in mammalian cells. Here, we study endocytic protein dynamics by live-cell imaging of the fluorescently tagged coat-associated protein Sla1-GFP and demonstrate that TL2 retains its inhibitory activity in S. cerevisiae spheroplasts, thus providing a unique tool for acute and reversible CME modulation in yeast.
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