Selected article for: "chromatography column and GE Healthcare size exclusion"
Author: Haixia Su; Sheng Yao; Wenfeng Zhao; Minjun Li; Jia Liu; Weijuan Shang; Hang Xie; Changqiang Ke; Meina Gao; Kunqian Yu; Hong Liu; Jingshan Shen; Wei Tang; Leike Zhang; Jianping Zuo; Hualiang Jiang; Fang Bai; Yan Wu; Yang Ye; Yechun Xu
Title: Discovery of baicalin and baicalein as novel, natural product inhibitors of SARS-CoV-2 3CL protease in vitro
  • Document date: 2020_4_14
    • ID: ixun0c8g_36
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038687 doi: bioRxiv preprint 3CLpro was inserted after the prescission protease digestion site of the PGEX6p-1 vector. The plasmid was transformed into BL21 (DE3) cells for protein expression. The expressed protein was purified by a GST column (GE Healthcare) followed by the size exclusion chromatography (Superdex200, GE Healthcare). T.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.13.038687 doi: bioRxiv preprint 3CLpro was inserted after the prescission protease digestion site of the PGEX6p-1 vector. The plasmid was transformed into BL21 (DE3) cells for protein expression. The expressed protein was purified by a GST column (GE Healthcare) followed by the size exclusion chromatography (Superdex200, GE Healthcare). The resulting protein was stored in a solution (25 mM Tris, pH 7.3) for the isothermal titration calorimetry measurements.

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