Selected article for: "1x Isothermal buffer and oligonucleotide mix"
Author: Sanchita Bhadra; Timothy E. Riedel; Miguel A. Saldaña; Shivanand Hegde; Nicole Pederson; Grant L. Hughes; Andrew D. Ellington
Title: Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone
  • Document date: 2018_3_29
    • ID: dbd2ndxa_20
    Snippet: LAMP reactions monitored in real time using OSD probes were assembled and analyzed as 195 above with the following changes. First, OSD probes were prepared by annealing 1 µM of the 196 fluorophore-labeled OSD strand with 5 µM of the quencher-labeled strand in 1X Isothermal buffer. Annealing was performed by denaturing the oligonucleotide mix at 95 °C for 1 min followed by 198 slow cooling at the rate of 0.1 °C/s to 25 °C. Excess annealed pro.....
    Document: LAMP reactions monitored in real time using OSD probes were assembled and analyzed as 195 above with the following changes. First, OSD probes were prepared by annealing 1 µM of the 196 fluorophore-labeled OSD strand with 5 µM of the quencher-labeled strand in 1X Isothermal buffer. Annealing was performed by denaturing the oligonucleotide mix at 95 °C for 1 min followed by 198 slow cooling at the rate of 0.1 °C/s to 25 °C. Excess annealed probe was stored at -20 °C. 199

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