Author: Sanchita Bhadra; Timothy E. Riedel; Miguel A. Saldaña; Shivanand Hegde; Nicole Pederson; Grant L. Hughes; Andrew D. Ellington
Title: Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone Document date: 2018_3_29
ID: dbd2ndxa_9
Snippet: Wolbachia wAlbB and wPip strain wsp genes and Ae. aegypti coi gene sequences were obtained 147 from NCBI GenBank. Consensus signature sequences were derived following MUSCLE 148 The Primer Explorer v5 primer design software (Eiken Chemical Co., Japan) was used for 154 generating several potential LAMP primer sets composed of the outer primers F3 and B3 and the 155 inner primers FIP and BIP. Primer design was constrained to include at least a 40 b.....
Document: Wolbachia wAlbB and wPip strain wsp genes and Ae. aegypti coi gene sequences were obtained 147 from NCBI GenBank. Consensus signature sequences were derived following MUSCLE 148 The Primer Explorer v5 primer design software (Eiken Chemical Co., Japan) was used for 154 generating several potential LAMP primer sets composed of the outer primers F3 and B3 and the 155 inner primers FIP and BIP. Primer design was constrained to include at least a 40 bp gap between 156 the F1 and F2 or between the B1 and B2 priming sites. Primer specificity for targeted sequence 157 and a corresponding lack of significant cross-reactivity to other nucleic acids of human, vector or 158 pathogenic origin were further assessed using NCBI BLAST. 159
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