Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies Document date: 2019_3_23
ID: 5bw7umap_72
Snippet: or CD8+ T cells were co-cultured with target cancer cells at a 1:1 ratio, with the addition of AICE at 0.075 OD550 x 200 μL medium (100 μL T cell medium + 100 μL cancer cell medium) (unless otherwise specified). Dual antigen (GFP and HER2) positive target cells (A375 and K562) were used as positive controls. After mixing, cells were centrifuged for 2 min at 300 x g to initiate interaction of the cells. After 24 hours, the co-culture from CD8+ .....
Document: or CD8+ T cells were co-cultured with target cancer cells at a 1:1 ratio, with the addition of AICE at 0.075 OD550 x 200 μL medium (100 μL T cell medium + 100 μL cancer cell medium) (unless otherwise specified). Dual antigen (GFP and HER2) positive target cells (A375 and K562) were used as positive controls. After mixing, cells were centrifuged for 2 min at 300 x g to initiate interaction of the cells. After 24 hours, the co-culture from CD8+ T cells were stained with anti-CD69 antibody (BD #562884) and analyzed by flow cytometry (BD LSR II), and also imaged through the spinning disk confocal microscope (Nikon Yokogawa CSU-22). After 48 hours, cytokine concentration in the supernatant was measured by IL-2 ELISA (for CD4+ T cells, eBiosciences #BMS2221HS) and Interferon-γ (IFN-γ) ELISA (for CD8+ T cells, ThermoFisher #KHC4021).
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