Author: Roberto Balbontín; Nelson Frazão; Isabel Gordo
Title: DNA breaks-mediated cost reveals RNase HI as a new target for selectively eliminating antibiotic resistance Document date: 2019_9_5
ID: 5hfenevk_25
Snippet: Fifteen independent colonies of sensitive bacteria were separately inoculated in LB in a 96-well plate, incubated at 37°C with shaking (700 r.p.m) for 7 hours, and 0.1ml of either independent culture was plated onto a LB agar plate supplemented with 150 µg/ml erythromycin, and incubated at 37°C for 5 days (Erm R strains grow in the presence of erythromycin, albeit slowly). Colonies able to grow in these plates were streaked onto plates supplem.....
Document: Fifteen independent colonies of sensitive bacteria were separately inoculated in LB in a 96-well plate, incubated at 37°C with shaking (700 r.p.m) for 7 hours, and 0.1ml of either independent culture was plated onto a LB agar plate supplemented with 150 µg/ml erythromycin, and incubated at 37°C for 5 days (Erm R strains grow in the presence of erythromycin, albeit slowly). Colonies able to grow in these plates were streaked onto plates supplemented with 150 µg/ml erythromycin, in order to further assess their bona fide resistance, and the rplD, and rplV genes of the resistant clones were amplified by PCR and analyzed by Sanger sequencing.
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