DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies

Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai

As an alternative to the standard protocol of supplementing free IL-2 in the media for ex vivo T cell culture 25 , we loaded IL-2 on AICE through the surface presentation via its antibody clone #5355 (Fig. Supplementary 3e) . This particular anti-IL-2 antibody was engineered to facilitate the binding of IL-2 to its β and γ receptor on T cells thus promoting the proliferation of non-Treg T cells 41 (Fig. 3g and Fig. Supplementary 3f ). Using the optimal condition of AICE[3:1] (anti-CD3:anti-CD28) determined above, we compared primary T cell expansion by supplementing equal amount of free IL-2 versus surface bound IL-2 ( Fig. Supplementary 3e) . Surface IL-2 loading enhanced CD4+ and CD8+ T cell expansion, and particularly improved expansion of CD4+ primary T cells after day 8 (Fig. 3h,i and The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/587105 doi: bioRxiv preprint 3a,g). Although with higher level of cell yield, the populations of LAG-3 and PD-1 positive CD4+ T cells still decreased in the two tested donors (Fig. Supplementary 3h ), demonstrating the advantages of AICE-surface presentation of IL-2 for ex vivo expansion.