Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies Document date: 2019_3_23
ID: 5bw7umap_60
Snippet: Complementary strands to the DNA scaffolds that were pre-conjugated with small molecules (e.g. fluorescent dye or biotin, at 1 μM/OD550) or proteins (e.g. GFP or antibodies, at 180 nM/OD550), were incubated with particles at 37 o C for 30 minutes in PBS buffer with 600 mM Na + and 0.01% Tween-20 supplemented for the surface hybridization of payloads. The excess was removed by three washes. For the coloading of multiple cargos, the input proporti.....
Document: Complementary strands to the DNA scaffolds that were pre-conjugated with small molecules (e.g. fluorescent dye or biotin, at 1 μM/OD550) or proteins (e.g. GFP or antibodies, at 180 nM/OD550), were incubated with particles at 37 o C for 30 minutes in PBS buffer with 600 mM Na + and 0.01% Tween-20 supplemented for the surface hybridization of payloads. The excess was removed by three washes. For the coloading of multiple cargos, the input proportion of each individual one with a specific sequence was consistent with its DNA-polymer counterpart input during the particle fabrication.
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