Author: Xiao Huang; Jasper Z. Williams; Ryan Chang; Zhongbo Li; Eric Gai; David M. Patterson; Yu Wei; Wendell A. Lim; Tejal A. Desai
Title: DNA-scaffolded biomaterials enable modular and tunable control of cell-based cancer immunotherapies Document date: 2019_3_23
ID: 5bw7umap_61
Snippet: For the loading of biotinylated biomolecules on 3'-biotinylated complementary DNA hybridized particles, a large excess of streptavidin (Prozyme #SA10) was added at 1.1 mg/mL per OD550 and incubated at room temperature for 30 mins, followed by three washes. Biotinylated antibody, protein or peptide was added at 180 nM/OD550 and incubated at room temperature for 30 mins to bind with surface streptavidin followed by three washes......
Document: For the loading of biotinylated biomolecules on 3'-biotinylated complementary DNA hybridized particles, a large excess of streptavidin (Prozyme #SA10) was added at 1.1 mg/mL per OD550 and incubated at room temperature for 30 mins, followed by three washes. Biotinylated antibody, protein or peptide was added at 180 nM/OD550 and incubated at room temperature for 30 mins to bind with surface streptavidin followed by three washes.
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