Author: Haixia Su; Sheng Yao; Wenfeng Zhao; Minjun Li; Jia Liu; Weijuan Shang; Hang Xie; Changqiang Ke; Meina Gao; Kunqian Yu; Hong Liu; Jingshan Shen; Wei Tang; Leike Zhang; Jianping Zuo; Hualiang Jiang; Fang Bai; Yan Wu; Yang Ye; Yechun Xu
Title: Discovery of baicalin and baicalein as novel, natural product inhibitors of SARS-CoV-2 3CL protease in vitro Document date: 2020_4_14
ID: ixun0c8g_44
Snippet: The protein sample of SARS-CoV-2-3CLpro was buffer exchanged with 10 mM ammonium acetate buffer (pH 6.8). For optimization of the MS condition, the protein was diluted into 2 μM. For ligand binding screening studies and Kd determination, samples was prepared with the protein at 1 μM and ligands at a series of concentrations (50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 μM for baicalin and 10, 5, 2.5, 1.25, 0.63, 0.31, 0.16, 0.08 μM for baicalein)......
Document: The protein sample of SARS-CoV-2-3CLpro was buffer exchanged with 10 mM ammonium acetate buffer (pH 6.8). For optimization of the MS condition, the protein was diluted into 2 μM. For ligand binding screening studies and Kd determination, samples was prepared with the protein at 1 μM and ligands at a series of concentrations (50, 25, 12.5, 6.25, 3.13, 1.56, 0.78 μM for baicalin and 10, 5, 2.5, 1.25, 0.63, 0.31, 0.16, 0.08 μM for baicalein).
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