Author: Jonathon A. Siva-Jothy; Pedro F. Vale
Title: Dissecting genetic and sex-specific host heterogeneity in pathogen transmission potential Document date: 2019_8_14
ID: 0v5q4kp9_2
Snippet: vortexing. These samples were then frozen at -70°C, to await DCV quantification by 166 qPCR. For each combination of sex and genetic background over the three days vial 167 load and virus shedding was measured, n=7-15 flies were measured (Table S2-S4) . Chloroform extraction. Samples were thawed on ice for 30 minutes before being 172 incubated at room temperature for 5 minutes to allow dissociation of nucleo-protein 173 complex. Samples were the.....
Document: vortexing. These samples were then frozen at -70°C, to await DCV quantification by 166 qPCR. For each combination of sex and genetic background over the three days vial 167 load and virus shedding was measured, n=7-15 flies were measured (Table S2-S4) . Chloroform extraction. Samples were thawed on ice for 30 minutes before being 172 incubated at room temperature for 5 minutes to allow dissociation of nucleo-protein 173 complex. Samples were then centrifuged at 12,000×g for 10 minutes at 4°C after 174 which large debris was removed. For phase separation, samples were shaken 175 vigorously for 15 seconds, 10μl of chloroform added, incubated at room temperature 176 for a further 3 minutes before being centrifuged at 12,000×g for 15 minutes at 4°C. 177
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