Transcriptomic dysregulations associated with SARS-CoV-2 infection in human nasopharyngeal and peripheral blood mononuclear cells

de Melo, Caroline Vilas Boas; Bhuiyan, Maruf Ahmed; Gatua, Winfred Nyoroka; Kanyerezi, Stephen; Uzairue, Leonard; Abechi, Priscilla; Kumar, Karan; Rahmat, Jabale; Giwa, Abdulazeez; Mwandira, Gracious; Olamilekan, Abisogun Mujib; George, Tiffany Ezinne; Adejinmi, Oluwapelumi John; Ibironke, Monsurat Ademidun; Rotimi, Olayemi David; Abo-Elenein, Dina Aly Mahmoud Aly; Abubakar, Ridwanullah Abiodun; Usman, Mahmood; Adewunmi, Ifeoluwa; Akinpelu, Oyewumi; Emmanuel, Olajide; Reang, Khatendra; Olalekan, Akadiri; Carl, Sarah H.

Introduction Over 24 million people have been infected globally with the novel coronavirus, SARS-CoV-2, with more than 820,000 succumbing to the resulting COVID-19 disease as of the end of August 2020. The molecular mechanisms underlying the pathogenesis of the disease are not completely elucidated. Thus, we aim to understand host response to SARS-CoV-2 infection by comparing samples collected from two distinct compartments (infection site and blood), obtained from COVID-19 subjects and healthy controls. Methods We used two publicly available gene expression datasets generated via RNA sequencing in two different samples; nasopharyngeal swabs and peripheral blood mononuclear cells (PBMCs). We performed a differential gene expression analysis between COVID-19 subjects and healthy controls in the two datasets and then functionally profiled their differentially expressed genes (DEGs). The genes involved in innate immunity were also determined. Results We found a clear difference in the host response to SARS-CoV-2 infection between the two sample groups. In COVID-19 subjects, the nasopharyngeal sample group indicated upregulation of genes involved in cytokine activity and interferon signalling pathway, as well as downregulation of genes involved in oxidative phosphorylation and viral transcription. Host response in COVID-19 subjects for the PBMC group, involved upregulation of genes involved in the complement system and immunoglobulin mediated immune response. CXCL13, GABRE, IFITM3 were upregulated and HSPA1B was downregulated in COVID-19 subjects in both sample groups. Conclusion Our results indicate the host response to SARS-CoV-2 is compartmentalized and suggests potential biomarkers of response to SARS-CoV-2 infection. Highlights Transcriptomic profiling from publicly available RNA-seq count data revealed a site-specific immune response in COVID-19. Host response was found cellular-mediated in nasopharyngeal samples and humoral-mediated in PBMCs samples. CXCL13, GABRE and IFITM3 commonly upregulated and HSPA1B downregulated in both sample groups highlights the potential of these molecules as markers of response to SARS-CoV-2 infection.