Author: Matteo De Chiara; Benjamin Barré; Karl Persson; Amadi Onyetuga Chioma; Agurtzane Irizar; Joseph Schacherer; Jonas Warringer; Gianni Liti
Title: Domestication reprogrammed the budding yeast life cycle Document date: 2020_2_9
ID: hwvr0v8c_15
Snippet: We next investigated the ability of wild yeasts to sporulate under the most extreme starvation, i.e. in water. We developed a high-throughput flow cytometry assay based on cell size and granularity (see methods and Fig. 2c ) to detect single (monad) and double (dyad) spores, as the former are not easily distinguished from unsporulated cells by microscopy......
Document: We next investigated the ability of wild yeasts to sporulate under the most extreme starvation, i.e. in water. We developed a high-throughput flow cytometry assay based on cell size and granularity (see methods and Fig. 2c ) to detect single (monad) and double (dyad) spores, as the former are not easily distinguished from unsporulated cells by microscopy.
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